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FULL-LENGTH SIL IGF-1 INTERNAL STANDARD

IGF-1 quantification starts before the instrument.

Evaluate a full-length stable isotope-labeled recombinant IGF-1 internal standard in your quantitative LC-MS/MS workflow.

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For clinical mass spectrometry, reference laboratories, and assay development teams.

PROTEIN-LEVEL PAIRSCHEMATIC · NOT STRUCTURAL DATA
Endogenous IGF-1LIGHT
Full-length SIL IGF-1HEAVY
A protein-level comparator designed for mass-distinguishable measurement. Behavior in the intended matrix and method requires evaluation.
Current flagshipIGF-1Product overview ↗
WHY THE ENTRY POINT MATTERS

Internal standardization begins where the standard enters.

A protein-level standard introduced before relevant processing and digestion can accompany more of the analytical workflow.

Explore the analytical rationale ↗
  1. 01
    SampleDefine matrix and target
  2. 02
    Add full-length SIL proteinBefore relevant processing
  3. 03
    Process / extractMethod-specific steps
  4. 04
    DigestFor bottom-up methods
  5. 05
    LCSeparate peptides
  6. 06
    MS detectionMeasure light / heavy
  7. 07
    QuantificationApply method calibration

A standard cannot account for changes before its addition. Method fit depends on the matrix, analyte behavior, and assay design.

TWO POINTS OF ADDITION

Full-length SIL protein vs heavy peptide.

Both support downstream LC-MS/MS measurement; they enter the workflow at different stages.

01 / EARLIER ENTRY

Full-length SIL protein

  1. Sample
  2. Add SIL protein standard enters
  3. Process / extract
  4. Digest
  5. LC
  6. MS detection
  7. Quantification

Can accompany more of the analytical processing when added before those steps.

02 / LATER ENTRY

Heavy peptide

  1. Sample
  2. Process / extract
  3. Digest
  4. Add heavy peptide standard enters
  5. LC
  6. MS detection
  7. Quantification

Helps normalize downstream measurement; the peptide itself does not undergo protein processing or digestion.

Earlier introduction allows a full-length SIL protein to share more analytical steps with the endogenous analyte. The extent of process control depends on the addition point and method.

01 / CURRENT PRODUCT
AVAILABLE FOR EVALUATION

Full-length SIL recombinant IGF-1.

The current flagship material for quantitative IGF-1 LC-MS/MS method development. Review its fit in your intended matrix and preparation route.

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PRODUCTION TECHNOLOGY

Chloroplast-based recombinant protein production.

Lite Lab's platform direction connects chloroplast engineering, controlled biomass generation, protein expression, and downstream processing. Each target requires its own development and characterization.

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Target geneChloroplast transformationControlled biomassProtein recovery
CUSTOM DEVELOPMENT

Bring us a protein target.

Discuss recombinant protein development around a defined use case and material requirements.

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APPLICATIONS

Follow the method.

Explore use in quantitative protein LC-MS/MS development and related analytical programs.

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NEXT STEP

Evaluate IGF-1 in your workflow

Tell us about your assay, matrix, and evaluation requirements. We can align the next technical discussion to your method.

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